Journal: Journal of Clinical Investigation

Article Title: T cell receptor grafting allows virological control of hepatitis B virus infection

doi: 10.1172/jci120228

Figure Lengend Snippet: Figure 4. Antiviral activity of different TCR-grafted T cell subsets. HepG2-NTCP cells were infected with HBV at a MOI of 500. After 2 to 3 weeks, T cells grafted with the TCRs 4GS20 (blue squares) or 6KC18 (red triangles) or nontransduced T cells (mock, gray circles) were added at decreasing E/T ratios. (A and B) Killing of target cells determined by detachment from the bottom of the 96-well plate was measured in real-time (xCELLigence) and is given as the normalized cell index relative to the starting point of the coculture with CD4+CD8+ T cells (A) or CD4+ T cells only (B). (C–F) CD8+ and CD4+ T cells were separated by positive magnetic cell sorting and added at an E/T ratio of 1:2. Cytokine-blocking antibodies against IFN-γ (10 ng/ml) or TNF-α (5 ng/ml) were given every other day when medium was exchanged. (C and D) IFN-γ and TNF-α were measured in the cell culture medium after 2 days. (E) Secreted HBeAg and (F) intracellular HBV cccDNA levels were measured after 10 days of coculture. Data are presented as mean values (A and B) or mean values ± SEM (C–F) of triplicate cocultures (n = 3).

Article Snippet: Cytokine-blocking antibodies against IFN-γ (10 ng/ml, clone B27, no. 506513, BioLegend) or TNF-α (5 ng/ml, clone D1B4, no. 7321, Cell Signaling Technology) were given every other day when medium was exchanged.

Techniques: Activity Assay, Infection, FACS, Blocking Assay, Cell Culture

Journal: Open Life Sciences

Article Title: Dissecting the molecular mechanisms of T cell infiltration in psoriatic lesions via cell-cell communication and regulatory network analysis

doi: 10.1515/biol-2025-1231

Figure Lengend Snippet: CuE alleviates IMQ-induced acute psoriasis in mice. (A) Photographs showing representative changes in mouse dorsal skin lesions; (B) psoriasis area and severity index (PASI) scores assessing psoriasis severity; (C) hematoxylin and eosin (H&E) staining showing histopathological changes in skin lesions. The overview images were captured at ×1 (scale bar 0.5 µm) and high-power images at ×200 (scale bar 20 µm); (D) quantification of epidermal thickness at lesion sites (μm); (E) immunohistochemistry (IHC) for Ki-67 expression; (F) number of Ki-67-positive cells in the epidermis. The overview images were captured at ×1 (scale bar 0.5 µm) and high-power images at ×200 (scale bar 20 µm); (G) western blot analysis of TNF-α, IL-6, and IL-1β protein levels. ** p < 0.01, *** p < 0.001 versus control group; ## p < 0.01, ### p < 0.001 versus model group.

Article Snippet: The membranes were incubated overnight at 4 °C with the following primary antibodies: IL-6 (1:1,000, 26404-1-AP, Proteintech, Wuhan, China); TNF-α (1:1,000, 17590-1-AP, Proteintech, Wuhan, China); IL-1β (1:2,000, 16806-1-AP, Proteintech, Wuhan, China); MDK (1:1,000, #DF6054, Affinity Biosciences, Jiangsu, China); P-STAT3 (1:1,000, #AF3293, Affinity Biosciences, Jiangsu, China); STAT3 (1:1,000, #AF6294, Affinity Biosciences, Jiangsu, China).

Techniques: Staining, Immunohistochemistry, Expressing, Western Blot, Control